WEKO3
アイテム
{"_buckets": {"deposit": "bf67d779-2ec0-44b3-9271-396830390d51"}, "_deposit": {"created_by": 3, "id": "682", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "682"}, "status": "published"}, "_oai": {"id": "oai:phoenix.repo.nii.ac.jp:00000682", "sets": ["50"]}, "author_link": ["994", "1006", "654", "988", "657", "658", "655", "998", "1578", "1572", "1584", "656", "1573", "1010", "653", "1002", "1004", "996"], "item_1_biblio_info_14": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-03-25", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "185", "bibliographicPageStart": "179", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{"bibliographic_title": "九州保健福祉大学研究紀要"}, {"bibliographic_title": "Journal of Kyushu University of Health and Welfare", "bibliographic_titleLang": "en"}]}]}, "item_1_creator_6": {"attribute_name": "著者名(日)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "定金, 豊"}, {"creatorName": "サダカネ, ユタカ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "988", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000060293304", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000060293304"}, {"nameIdentifier": "1000060293304", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "60293304", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60293304"}]}, {"creatorNames": [{"creatorName": "北本, 英二郎"}, {"creatorName": "キタモト, エイジロウ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "1572", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006546373", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006546373"}]}, {"creatorNames": [{"creatorName": "友廣, 岳則"}, {"creatorName": "トモヒロ, タケノリ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "1573", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006052243", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006052243"}]}, {"creatorNames": [{"creatorName": "木葉, 敬子"}, {"creatorName": "コノハ, ケイコ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "653", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000000036175", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000000036175"}]}, {"creatorNames": [{"creatorName": "川原, 正博"}, {"creatorName": "カワハラ, マサヒロ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "654", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040224828", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000040224828"}, {"nameIdentifier": "1000040224828", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "40224828", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40224828"}]}, {"creatorNames": [{"creatorName": "畑中, 保丸"}, {"creatorName": "ハタナカ, ヤスマル", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "994", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_7": {"attribute_name": "著者名よみ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "サダカネ, ユタカ"}], "nameIdentifiers": [{"nameIdentifier": "996", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000060293304", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000060293304"}]}, {"creatorNames": [{"creatorName": "キタモト, エイジロウ"}], "nameIdentifiers": [{"nameIdentifier": "1578", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006546373", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006546373"}]}, {"creatorNames": [{"creatorName": "トモヒロ, タケノリ"}], "nameIdentifiers": [{"nameIdentifier": "998", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006052243", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006052243"}]}, {"creatorNames": [{"creatorName": "コノハ, ケイコ"}], "nameIdentifiers": [{"nameIdentifier": "655", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000000036175", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000000036175"}]}, {"creatorNames": [{"creatorName": "カワハラ, マサヒロ"}], "nameIdentifiers": [{"nameIdentifier": "656", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040224828", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000040224828"}]}, {"creatorNames": [{"creatorName": "ハタナカ, ヤスマル"}], "nameIdentifiers": [{"nameIdentifier": "1002", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_8": {"attribute_name": "著者名(英)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "SADAKANE, Yutaka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1004", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000060293304", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000060293304"}]}, {"creatorNames": [{"creatorName": "KITAMOTO, Eiziro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1584", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006546373", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006546373"}]}, {"creatorNames": [{"creatorName": "TOMOHIRO, Takenori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1006", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006052243", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006052243"}]}, {"creatorNames": [{"creatorName": "KONOHA, Keiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "657", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000000036175", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000000036175"}]}, {"creatorNames": [{"creatorName": "KAWAHARA, Masahiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "658", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040224828", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000040224828"}]}, {"creatorNames": [{"creatorName": "HATANAKA, Yasumaru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1010", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_description_1": {"attribute_name": "ページ属性", "attribute_value_mlt": [{"subitem_description": "P(論文)", "subitem_description_type": "Other"}]}, "item_1_description_11": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "オピオイド結合細胞接着分子(OBCAM)タンパク質は、イムノグロブリンのスーパーファミリーに属し、エンケファリンやβ-エンドルフィンなどのオピオイドリガンドを認識する。我々は、大腸菌で組換えOBCAMタンパク質と、β-エンドルフィンの部分配列をもとにジアジリンとビオチンをもつ光アフィニティーリガンドとを製作し、光アフィニティーラベル実験を行った。光照射により両者が共有結合で結ばれた複合体を形成した。しかしながら、[Leu]-エンケファリン,[D-Ala^2, D-Leu^5]-エンケファリンおよびβ-エンドルフィンを過剰に加えても、この複合体形成を阻害できなかった。光アフィニティーリガンドと同一の構造をもつ(ジアジリンをもつ)ペプチドアナログのみが複合体形成を阻害した。このペプチドアナログ中のジアジリンを光分解したものは阻害活性を失った。これらの結果は、オピオイドペプチド中のジアジリン構造がOBCAMタンパク質との親和性を増強させていることを示している。", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "An opioid-binding cell-adhesion molecule (OBCAM) protein, which is an immunoglobulin superfamily protein, recognizes various opioid ligands including enkephalin and β-endorphin. The molecular mechanisms of ligand-binding interface of the proteins are not yet clear. We prepared a bacterial expressed OBCAM protein using pGEX4T expression system, and photoaffinity ligand bearing diazirine and biotin, which was constructed using the partial sequence of β-endorphin, Tyr-Gly-Gly-Phe-Leu-Thr-Ser-Glu-Lys-Ser. Crosslinking formation between OBCAM protein and photoaffinity ligand was confirmed by SDS-PAGE analysis. Neither excess amount of [Leu]-enkephalin, [D-Ala^2, D-Leu^5]-enkephalin, nor β-endorphin inhibited the crosslinking formation. The diazirine-bearing peptide that had an identical amino acid sequence to that of the photoaffinity ligand only inhibited the crosslinking formation, and the removal of diazirine on the peptide by photolysis resulted in loss of the inhibitory activity. These results show that diazirine group on the opioid ligand enhanced its binding affinity to the OBCAM protein.", "subitem_description_type": "Other"}]}, "item_1_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.15069/00000676", "subitem_identifier_reg_type": "JaLC"}]}, "item_1_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11490417", "subitem_source_identifier_type": "NCID"}]}, "item_1_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Faculty of Pharmaceutical Sciences, Kyushu University of Health and Welfare"}, {"subitem_text_language": "en", "subitem_text_value": "Faculty of Pharmaceutical Sciences, University of Toyama"}, {"subitem_text_language": "en", "subitem_text_value": "Faculty of Pharmaceutical Sciences, University of Toyama"}, {"subitem_text_language": "en", "subitem_text_value": "Faculty of Pharmaceutical Sciences, Kyushu University of Health and Welfare"}, {"subitem_text_language": "en", "subitem_text_value": "Faculty of Pharmaceutical Sciences, Kyushu University of Health and Welfare"}, {"subitem_text_language": "en", "subitem_text_value": "Faculty of Pharmaceutical Sciences, University of Toyama"}]}, "item_1_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "九州保健福祉大学薬学部"}, {"subitem_text_value": "富山大学薬学部"}, {"subitem_text_value": "富山大学薬学部"}, {"subitem_text_value": "九州保健福祉大学薬学部"}, {"subitem_text_value": "九州保健福祉大学薬学部"}, {"subitem_text_value": "富山大学薬学部"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2007-03-25"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "KJ00004702918.pdf", "filesize": [{"value": "705.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 705800.0, "url": {"label": "KJ00004702918", "url": "https://phoenix.repo.nii.ac.jp/record/682/files/KJ00004702918.pdf"}, "version_id": "cb254889-836c-4dbd-b53f-d7bcb0b85acc"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "光反応基", "subitem_subject_scheme": "Other"}, {"subitem_subject": "β-エンドルフィン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "リガンド-受容体インターフェイス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "photoreactive group", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "β-endorphin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ligand-receptor interface", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ジアジリン型光反応性リガンドによるオピオイド結合細胞接着分子(OBCAM)の光アフィニティーラベリング", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ジアジリン型光反応性リガンドによるオピオイド結合細胞接着分子(OBCAM)の光アフィニティーラベリング"}, {"subitem_title": "Photoaffinity labeling of opioid-binding cell-adhesion molecule (OBCAM) by phenyldiazirine-integrated ligands", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "3", "path": ["50"], "permalink_uri": "https://doi.org/10.15069/00000676", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-03-25"}, "publish_date": "2007-03-25", "publish_status": "0", "recid": "682", "relation": {}, "relation_version_is_last": true, "title": ["ジアジリン型光反応性リガンドによるオピオイド結合細胞接着分子(OBCAM)の光アフィニティーラベリング"], "weko_shared_id": 3}
ジアジリン型光反応性リガンドによるオピオイド結合細胞接着分子(OBCAM)の光アフィニティーラベリング
https://doi.org/10.15069/00000676
https://doi.org/10.15069/000006765ff48e73-93c4-4a50-b9b3-9a1501049cec
名前 / ファイル | ライセンス | アクション |
---|---|---|
KJ00004702918 (705.8 kB)
|
Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2007-03-25 | |||||||||
タイトル | ||||||||||
タイトル | ジアジリン型光反応性リガンドによるオピオイド結合細胞接着分子(OBCAM)の光アフィニティーラベリング | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Photoaffinity labeling of opioid-binding cell-adhesion molecule (OBCAM) by phenyldiazirine-integrated ligands | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
主題Scheme | Other | |||||||||
主題 | 光反応基 | |||||||||
キーワード | ||||||||||
主題Scheme | Other | |||||||||
主題 | β-エンドルフィン | |||||||||
キーワード | ||||||||||
主題Scheme | Other | |||||||||
主題 | リガンド-受容体インターフェイス | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | photoreactive group | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | β-endorphin | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | ligand-receptor interface | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | departmental bulletin paper | |||||||||
ID登録 | ||||||||||
ID登録 | 10.15069/00000676 | |||||||||
ID登録タイプ | JaLC | |||||||||
ページ属性 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | P(論文) | |||||||||
著者名(日) |
定金, 豊
× 定金, 豊× 北本, 英二郎× 友廣, 岳則× 木葉, 敬子× 川原, 正博
WEKO
654
× 畑中, 保丸 |
|||||||||
著者名よみ |
サダカネ, ユタカ
× サダカネ, ユタカ× キタモト, エイジロウ× トモヒロ, タケノリ× コノハ, ケイコ× カワハラ, マサヒロ× ハタナカ, ヤスマル |
|||||||||
著者名(英) |
SADAKANE, Yutaka
× SADAKANE, Yutaka× KITAMOTO, Eiziro× TOMOHIRO, Takenori× KONOHA, Keiko× KAWAHARA, Masahiro× HATANAKA, Yasumaru |
|||||||||
著者所属(日) | ||||||||||
値 | 九州保健福祉大学薬学部 | |||||||||
著者所属(日) | ||||||||||
値 | 富山大学薬学部 | |||||||||
著者所属(日) | ||||||||||
値 | 富山大学薬学部 | |||||||||
著者所属(日) | ||||||||||
値 | 九州保健福祉大学薬学部 | |||||||||
著者所属(日) | ||||||||||
値 | 九州保健福祉大学薬学部 | |||||||||
著者所属(日) | ||||||||||
値 | 富山大学薬学部 | |||||||||
著者所属(英) | ||||||||||
言語 | en | |||||||||
値 | Faculty of Pharmaceutical Sciences, Kyushu University of Health and Welfare | |||||||||
著者所属(英) | ||||||||||
言語 | en | |||||||||
値 | Faculty of Pharmaceutical Sciences, University of Toyama | |||||||||
著者所属(英) | ||||||||||
言語 | en | |||||||||
値 | Faculty of Pharmaceutical Sciences, University of Toyama | |||||||||
著者所属(英) | ||||||||||
言語 | en | |||||||||
値 | Faculty of Pharmaceutical Sciences, Kyushu University of Health and Welfare | |||||||||
著者所属(英) | ||||||||||
言語 | en | |||||||||
値 | Faculty of Pharmaceutical Sciences, Kyushu University of Health and Welfare | |||||||||
著者所属(英) | ||||||||||
言語 | en | |||||||||
値 | Faculty of Pharmaceutical Sciences, University of Toyama | |||||||||
抄録(日) | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | オピオイド結合細胞接着分子(OBCAM)タンパク質は、イムノグロブリンのスーパーファミリーに属し、エンケファリンやβ-エンドルフィンなどのオピオイドリガンドを認識する。我々は、大腸菌で組換えOBCAMタンパク質と、β-エンドルフィンの部分配列をもとにジアジリンとビオチンをもつ光アフィニティーリガンドとを製作し、光アフィニティーラベル実験を行った。光照射により両者が共有結合で結ばれた複合体を形成した。しかしながら、[Leu]-エンケファリン,[D-Ala^2, D-Leu^5]-エンケファリンおよびβ-エンドルフィンを過剰に加えても、この複合体形成を阻害できなかった。光アフィニティーリガンドと同一の構造をもつ(ジアジリンをもつ)ペプチドアナログのみが複合体形成を阻害した。このペプチドアナログ中のジアジリンを光分解したものは阻害活性を失った。これらの結果は、オピオイドペプチド中のジアジリン構造がOBCAMタンパク質との親和性を増強させていることを示している。 | |||||||||
抄録(英) | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | An opioid-binding cell-adhesion molecule (OBCAM) protein, which is an immunoglobulin superfamily protein, recognizes various opioid ligands including enkephalin and β-endorphin. The molecular mechanisms of ligand-binding interface of the proteins are not yet clear. We prepared a bacterial expressed OBCAM protein using pGEX4T expression system, and photoaffinity ligand bearing diazirine and biotin, which was constructed using the partial sequence of β-endorphin, Tyr-Gly-Gly-Phe-Leu-Thr-Ser-Glu-Lys-Ser. Crosslinking formation between OBCAM protein and photoaffinity ligand was confirmed by SDS-PAGE analysis. Neither excess amount of [Leu]-enkephalin, [D-Ala^2, D-Leu^5]-enkephalin, nor β-endorphin inhibited the crosslinking formation. The diazirine-bearing peptide that had an identical amino acid sequence to that of the photoaffinity ligand only inhibited the crosslinking formation, and the removal of diazirine on the peptide by photolysis resulted in loss of the inhibitory activity. These results show that diazirine group on the opioid ligand enhanced its binding affinity to the OBCAM protein. | |||||||||
雑誌書誌ID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA11490417 | |||||||||
書誌情報 |
九州保健福祉大学研究紀要 en : Journal of Kyushu University of Health and Welfare 巻 8, p. 179-185, 発行日 2007-03-25 |