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血管内皮前駆細胞から分泌される可溶性CD93分子
https://doi.org/10.15069/00000960
https://doi.org/10.15069/000009604e9d72ff-cc80-4fee-a010-19ff7d5f40af
名前 / ファイル | ライセンス | アクション |
---|---|---|
KJ00009811239 (2.7 MB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-03-25 | |||||
タイトル | ||||||
タイトル | 血管内皮前駆細胞から分泌される可溶性CD93分子 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Secretion of soluble-form CD93 from human endothelial progenitor cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | サイトカラシンE | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ヒト血管内皮前駆細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ホルボールミリステートアセテート | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 可溶性CD93 分子 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cytochalasin E | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | human endothelial progenitor cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phorbol 12-myristate 13-acetate | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | soluble-form CD93 molecule | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15069/00000960 | |||||
ID登録タイプ | JaLC | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
著者名(日) |
池脇, 信直
× 池脇, 信直× 園田, 徹× 土井, 宏太郎× 大野, 英治 |
|||||
著者名よみ |
イケワキ, ノブナオ
× イケワキ, ノブナオ× ソノダ, トオル× ドイ, コウタロウ× オオノ, エイジ |
|||||
著者名(英) |
IKEWAKI, Nobunao
× IKEWAKI, Nobunao× SONODA, Tohru× DOI, Koutarou× OHNO, Eiji |
|||||
著者所属(日) | ||||||
値 | 九州保健福祉大学薬学部動物生命薬科学科臨床免疫学研究室 | |||||
著者所属(日) | ||||||
値 | 九州保健福祉大学保健科学部作業療法学科 | |||||
著者所属(日) | ||||||
値 | 宮崎県立延岡病院産婦人科 | |||||
著者所属(日) | ||||||
値 | 九州保健福祉大学QOL 研究機構 | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Laboratory of Clinical Immunology, Department of Animal Pharmaceutical Science, Kyushu University of Health and Welfare School of Pharmaceutical Sciences | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Department of Occupational Therapy, Kyushu University of Health and Welfare School of Health Science | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Department of Obstetrics and Gynecology, Miyazaki Prefectural Nobeoka Hospital | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Quality of Life Research Organization, Kyushu University of Health and Welfare | |||||
抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | ヒト血管内皮前駆細胞(Human endothelial progenitor cells :HEPCs)から分泌される可溶性CD93(sCD93)分子の動態を自主開発した抗ヒトCD93 抗体(mNI-11)を用いて解析した。HEPCs は低濃度であるが、sCD93 分子を培養液中に自然分泌していることがわかった。HEPCs をPKC 活性剤であるPMAで処理したところ、sCD93 分子の分泌は有意に増強した(P < 0.01)。また、PMA 処理の初期段階からsCD93 分泌の増強は認められた。次に、PMA 処理HEPCs にPKC 活性阻害剤であるGo6976 を添加したところ、sCD93 分子の分泌は有意に抑制された(P < 0.01)。一方、HEPCs をアクチンフィラメントの脱重合剤(サイトカラシンE)で処理したところ、sCD93 分子の分泌が有意に増強した(P < 0.01)。HEPCs から分泌されるsCD93 分子は、PKC によるリン酸化とアクチンフィラメントの脱重合が密接に関与していることがわかった。 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | In this study, we examined the secretion of soluble-form CD93 (sCD93) from human endothelial progenitor cells (HEPCs) expressing cell surface CD93 by enzyme-linked immunoassay (EIA) using CD93 monoclonal antibody (mAb) (mNI-11) established in our laboratories. We found that a small amount of sCD93 was spontaneously secreted into the culture supernatants of HEPCs. Furthermore, significantly (P < 0.01) enhanced secretion of sCD93 was found in the culture supernatants of HEPCs treated with phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, which was significantly (P < 0.01) suppressed by treatment of the cells with the protein kinase (PKC) inhibitor Go6976. Interestingly, depolymerization of the actin filaments in the cells by cytochalasin E (CyE) also significantly (P < 0.01) enhanced the sCD93 secretion into the culture supernatants of HEPCs. Taken together, these findings suggest that the sCD93 might serve as a new biomarker for analyzing the biological and immunological functions of HEPCs. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11490417 | |||||
書誌情報 |
九州保健福祉大学研究紀要 en : Journal of Kyushu University of Health and Welfare 巻 16, p. 121-128, 発行日 2015-03 |